Studies an chromatin. II. Isolation and characterization of chromatin subunits
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چکیده
منابع مشابه
Isolation and partial characterization of dinoflagellate chromatin.
Chromatin was prepared by two dif ferent methods f rom isolated nuclei of Gyrodinium cohnii ( Cryptothecodinium cohnii) and Peridinium trochoideum. These isolation procedures are different from those generally used to prepare eukaryote chromatin, because the latter do not work for dinoflagellate chromatin. The chemical composition of this chromatin is similar for both methods of preparation and...
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Evidence suggests that human sperm chromatin anomalies adversely affect reproductive outcomes and infertile men possess substantially amount of sperm with chromatin anomalies than fertile men. Routine semen analysis evaluates parameters such as sperm motility and morphology, but does not examine the nuclear DNA integrity of spermatozoa. It has been suggested that altered nuclear chromatin struc...
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Different preparations of chromatin isolated from mycelia of Neurospora crassa were analyzed for DNA-associated RNA and proteins. The UV absorption spectra, the ultrastructure of chromatin, and the amino acid composition of the acid-extractable proteins were studied. The protein:DNA ratios range from 1.5 to 2.8; the RNA:DNA ratios range from 0.5 to 1.24. UV absorption shows a macimum at 259 mmi...
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Nuclei were isolated from rat liver during the prolonged feeding of the 2 carcinogens, N-hydroxy^-acetylaminofluorene and 3'-methyl-4-dimethylaminoazobenzene, and during the pro longed feeding of the noncarcinogen, 2-methyl-4-dimethylaminoazobenzene. Nuclei were also isolated from rat liver tumors induced by the above carcinogens. Chromatin was isolated from these nuclei and analyzed for RNA, t...
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Partial digestion of rat liver nuclei with staphylococcal nuclease results in the liberation of nucleo-protein complexes consisting of one or more upsilon bodies. By velocity centrifugation we have isolated the monomeric subunit in relatively pure form. We find that this subunit contains 185 base pairs of DNA and 240,000 daltons of protein, resulting in a protein to DNA ratio identical to that ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1975
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/2.8.1401